Table 1.
List of reported antibiotic susceptibility assays.
| Group/reference | Definition of MIC and MBC/MCC/MLC | Cells | Species |
|---|---|---|---|
| Suchland et al., 2003 | MIC: “The transition point MIC (MICTP) was defined as the concentration of drug in which 90% or more of the inclusions were altered in size and morphology. The MIC was defined as the concentration of drug that is one twofold dilution more concentrated than the MICTP.” (p. 637) MCC: “The lowest concentration of drug that produced no morphologically normal inclusions by one freeze-thaw passage (…) in antimicrobial-free medium.” (p. 637) | HeLa, BGMK, HEp-2, Vero | CT1, Cpsi2, Cpne3 |
| Donati et al., 2010 | MIC: “The lowest concentration that reduced the number of inclusions more than 90% compared with the level for drug-free controls.” (p. 5380) MBC: “The MBC concentration was measured by aspirating the antibiotic-containing medium, washing the monolayer twice with PBS, and incubating it in (…) antibiotic-free medium for 48 h at 35°C. (…). The MBC was the lowest concentration of the drug reducing more than 90% demonstrable inclusions after monolayers were re-incubated in antimicrobial-free medium.” (p. 5380) | LLC-MK2 | CT1 |
| Japan Society of Chemotherapy, 1992a,b | MIC: “The lowest concentration, which completely inhibits inclusion body formation.” (p. 309) MLC: “The lowest concentration, which completely inhibits the reformation of chlamydial inclusion in infected HeLa 229 cells even after elimination of the drug from the culture medium (after 24 h).” (p. 318) | HeLa, McCoy | CT1, Cpsi2, Cpne3 |
| Hammerschlag, 1982; Ehret and Judson, 1988 | MIC: “The MIC should be defined as no inclusions seen.” (Ehret, p. 1298; Hammerschlag, p. 500) MBC: “(…) the MBC should be defined as no inclusion seen on passaging.” (Ehret, p. 1298) | McCoy | CT1 |
| Webberley et al., 1987 | MIC: “The MIC was defined as the lowest concentration of antibiotic that inhibited inclusion development after 48 h.” (p. 409) MLC: “(…) the lowest concentration that prevented the reappearance of inclusions after re-incubation in antibiotic free media.” (p. 409) | McCoy | Cpne3 |
| Agacfidan et al., 1993 | MIC: “The MIC for Chlamydia was defined as the concentration of antibiotic that allowed no inclusions on the first passage.” (p. 1993) MCC: “The MCC was defined as the lowest concentration that allowed no inclusions in a further passage in the absence of antibiotics.” (p. 1993) | McCoy | CT1 |
| Henning and Krauss, 1986 | MIC (MHK, Minimale Hemmkonzentration): “The lowest concentration where there are no inclusions.” (p. 448) MBC (Washed and reincubated, W & R): “The lowest concentration of drug that produced no inclusions after removal of the drug and several washing steps during incubation in drug-free medium.” (p. 448) | BGM, McCoy | Cpsi2 |
1C. trachomatis, 2C. psittaci, 3C. pneumoniae.