Skip to main content
. 2018 Jul 10;4:36. doi: 10.1038/s41421-018-0035-0

Fig. 4. catRNA enables efficient gene activation with catalytically dead LbCpf1 activators.

Fig. 4

a Schematic representation of using the dCpf1-VP64/VPR activator and crRNA/catRNA arrays for synergistic gene activation. Spa: Spacer. b Synergistic gene activation with the dCpf1-VP64 activator. Plasmids encoding single or multiple crRNAs or a crRNA array targeting the DNMT1 and VEGFa promoter regions were transfected together with the dCpf1-VP64 plasmid into 293T cells. Gene expression was measured by quantitative real-time PCR 48 h post-transfection. c Efficient gene activation was achieved with a single catRNA and the dCpf1-VPR activator. Single crRNA, caRNA, or catRNA targeting the DNMT1 and VEGFa promoter regions were co-transfected with the dCpf1-VPR fusion protein into 293T cells, and gene expression was calculated by quantitative real-time PCR 48 h post-transfection. Bar, SE. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 determined by the Mann–Whitney test