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. 2018 Jul 9;8:10360. doi: 10.1038/s41598-018-28511-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adult il10^e46/e46 zebrafish have enhanced survival compared to WT controls in a low-dose M. marinum infection. (a) il10^e46/e46 (n = 158) and WT (n = 181) zebrafish larvae were microinjected before 6 hours post fertilization with M. marinum (3–29 CFU) and their survival was monitored for 7 days. The experiment was done six times and the data presented here is collected from one representative experiment. (b) The survival of adult il10^e46/e46 (n = 172) and WT (n = 149) zebrafish was monitored for 16–20 weeks after a low-dose (2–156 CFU) mycobacterial infection. The data were collected from four experiments. (c) The M. marinum burden in abdominal organ blocks (including kidney in the first and the second experiment, without kidney in the third experiment) of adult il10^e46/e46 mutant zebrafish (n = 22–27) and WT controls (n = 22–30 fish) was quantified with qPCR at 4 and 8/9 weeks post a low-dose infection (1–18 CFU). The bacterial load is presented as a scatter dot plot and as the median of total bacterial copies (log₁₀). The data were collected from three experiments. (d) M. marinum granulomas were detected with Ziehl-Neelsen staining (n = 4 in both groups at both time points) at 4 and 9 weeks post a low-dose infection (2–9 CFU). Representative individuals from each group are shown. Granulomas are indicated with arrows. For panels (a) and (b) a log-rank (Mantel-Cox) and for panel (c) A two-tailed Mann-Whitney test was used for the statistical comparison of differences.