Fig. 3. GSH depletion does not induce mitochondrial superoxide or alter mitochondrial mass in RPE cells.

a Mitochondrial ROS assessed in ARPE-19 cells treated for 24 h with Cys₂ starvation, 1000 µM BSO, and 10 µM erastin by flow cytometry using MitoSOX Red. b Mitochondrial mass was measured by MitoTracker Red dye. Scale bars: 20 μm. (c) and (d) represent quantified data depicted in (a, b). Data in (c, d) represent mean ± SD from one of three representative experiments. Representative data from one of three experiments are shown