Fig. 7. GSH depletion arrests cell growth and induces premature cell senescence.

a Cell cycle analysis by PI staining in cells treated with Cys₂ starvation, 1000 µM BSO, and 10 µM erastin. (b) represent quantified data depicted in (a). Proportion of cells in G1 + S phase and G2 phase were calculated. Data represent mean ± SD from one of three representative experiments. Representative data from one of three experiments are shown. * represent p < 0.05, ** represent p < 0.01. c Images of senescence β-galactosidase staining. Cells were treated with Cys₂ starvation, 1000 µM BSO, and 10 µM erastin for 24 h, respectively. Scale bars: 50 μm. d GSH-depleted cells were stained with DAPI to show SAHFs. e Senescence-associated gene profiles of Cys₂ starved and 1000 µM BSO and 10 µM erastin-treated ARPE-19 cells. Total RNA was extracted at 24 h post treatment and assayed on BGISEQ-500 RNA-Seq