Table 2.
Nucleotide and inhibitor affinity of malaria and human GRP78.
| PfGRP78-NBD wt | PfGRP78-NBD mut | huGRP78-NBD | |
|---|---|---|---|
| ADP* | 16.2 ± 2.8 | 8.11 ± 0.73 | 0.49 ± 0.02 |
| ATP* | 333 ± 74 | 312 ± 15 | 14.0 ± 7.0 |
| Apoptozole | 4.27 ± 0.86 | N.B. | 6.9 ± 0.33 |
| MKT-077 | 16.4 ± 1.7 | — | 16 ± 0.17 |
| VER-155008 | 164 ± 13.6 | — | 52.7 ± 27 |
| Gilvocarcin V | N.B. | — | N.B. |
| ARL-67156 | N.B. | — | N.B. |
| Elesclomol | N.B. | — | N.B. |
The affinity constants (Kd) determined by SPR are indicated in µM plus/minus their standard error (SE). Statistically significant differences between the two PfGRP78 and the host huGRP78 by an * sign (p < 0.05). N.B. = No binding; “—“ = Not determined.