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. 2018 Jul 3;9:1279. doi: 10.3389/fmicb.2018.01279

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Copyright © 2018 Sinha, C, Raghwan, Das, Das, Shadab, Chowdhury, Tripathy and Ali.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Frameshift mutation in Calpain like cysteine protease gene due to a single insertion in late passage. A single insertion in HTI5 (as well as Genbank LDBPK 282 A1) completely changes the coding frame of the gene (Upper panel) making it inactive. The changes in late passage and Genbank strain is consistent.