Table 3.
List of proteins differentially expressed (≥1.5-folds) between early and late passages identified by MALDI-ToF MS/MS.
| Spot Number | Assembly gap? location∗ | Protein annotation | Accession number of protein | Protein score | Exp/Thr M.W | Exp/ Thr pI | aFold change (MS/MS) | bFold change Values using RNAseq |
|---|---|---|---|---|---|---|---|---|
| 1 | Yes | Constitutive major surface protease | CAC37962.1 | 434 | 68/63.8 | 5.9/6.84 | 0.66 | –0.67 |
| 2 | Yes | Constitutive major surface protease | CAC37962.1 | 393 | 68.5/63.8 | 5.7/6.84 | 0.45 | –0.42 |
| 3 | Yes | GP63, leishmanolysin | XP_001463701.2 | 75 | 69/63.8 | 5.6/6.84 | 0.05 | –0.42 |
| 4 | Yes | GP63, leishmanolysin | XP_001463701.2 | 114 | 40/63.8 | 5.4/6.84 | 0.1 | –0.42 |
| 5 | Yes | Beta tubulin | XP_003878331.1 | 347 | 55.8/49.7 | 5.2/4.71 | 6.38 | 0.22 |
| 6 | Yes | Putative heat shock 70-related protein 1, mitochondrial precursor | XP_003877392.1 | 271 | 68.5/63.8 | 5.8/6.84 | 0.23 | –0.46 |
| 8 | Chr36: 1099755–1098367 | Putative dihydrolipoamide acetyltransferase precursor | XP_001469441.1 | 382 | 62.4/48.62 | 6.4/7.02 | 0.44 | –0.76 |
| 9 | Yes | ∗∗Activated C kinase protein, partial | ABS82039.1 | 103 | 31.2/30.63 | 6.7/6.63 | 0.66 | –0.20 |
| 10 | Chr32: 766497–767468 | Putative heat shock protein-like protein | XP_001467884.1 | 308 | 22.5/35.49 | 5/4.94 | 0.32 | –0.24 |
| 11 | chr36: 2665342–2666118 | Conserved hypothetical protein | XP_001469385.1 | 703 | 24/29.12 | 5.4/5.82 | 0.24 | 0.54 |
| 12 | chr25: 350814–352157 | ∗∗Gamma tubulin | XP_003860740.1 | 274 | 22.9/49.7 | 5.2/4.71 | 0.28 | –0.16 |
| 13 | chr23: 9640–10320 | ∗∗Mitochondrial peroxiredoxin | AAX73294.1 | 314 | 20.6/25.34 | 5.4/6.43 | 0.27 | –0.82 |
| 14 | chr22: 569013–569636 | i/6 autoantigen-like protein | XP_001465646.1 | 156 | 26.2/23.01 | 6/5.68 | 0.37 | –0.03 |
| 15 | chr36: 1493409–1494356 | Hypothetical protein, conserved (MORN repeat) | XP_003865511.1 | 142 | 18.1/35.67 | 4.8/5.90 | 0.22 | –0.16 |
| 16 | chr35: 896773–897597 | Putative RNA-binding protein | XP_003392812.1 | 344 | 22.6/30.25 | 6.5/7.85 | 0.61 | –0.05 |
| 17 | chr20: 601988–602383 | Putative small myristoylated protein-1 | XP_001465265.1 | 56 | 17.4/12.94 | 6.4/4.70 | 1.96 | 0.56 |
| 18 | chr35: 552681–553127 | ∗∗Putative ubiquitin-conjugating enzyme E2 | XP_001568232.1 | 46 | 13.1/16.65 | 6.3/6.08 | 0.43 | –0.03 |
| 19 | chr23: 133149–134204 | Putative NADP-dependent alcohol dehydrogenase | XP_001465717.1 | 388 | 43.2/38.45 | 6.5/5.96 | 0.54 | –1.48 |
| 20 | chr25: 458908–460485 | Putative ATPase beta subunit | XP_001466152.1 | 211 | 60.5/56.32 | 5.5/5.14 | 2.06 | 0.62 |
| 21 | chr28: 488208–489566 | ∗∗Phenylalanine-4-hydroxylase | XP_001684410.1 | 71 | 48.8/51.48 | 6.2/5.93 | 0.37 | –0.21 |
| 22 | chr36: 2726362–2727795 | ∗∗Protein disulfide isomerase | XP_001469404.1 | 206 | 55.5/52.34 | 5.5/5.42 | 0.34 | –0.23 |
| 23 | chr25: 671470–672522 | Putative pyruvate dehydrogenase E1 beta subunit | XP_001466210.1 | 72 | 45.8/37.84 | 5.5/5.64 | 0.27 | –2.02 |
| 24 | Yes | Alpha tubulin | XP_003873239.1 | 113 | 14.9/49.75 | 4.8/4.89 | 0.35 | –0.04 |
| 25 | chr21: 9660–11141 | Conserved hypothetical protein | XP_001564720.1 | 55 | 23.4/52.12 | 5.6/6.45 | 0.43 | –0.13 |
| 26 | chr21: 413285–414697 | Alpha tubulin | XP_003873239.1 | 68 | 11.3/49.75 | 5.6/4.89 | 0.36 | –0.04 |
| 27 | chr7: 303267–303773 | Conserved hypothetical protein/putative Qa-SNARE protein | XP_003872214.1/ XP_001463282.1 | 59 | 11.5/14.35 | 5.2/9.15 | 0.52 | –1.24 |
| 28 | Yes | Conserved hypothetical protein | XP_001470159.1 | 82 | 33.5/24.26 | 5.6/5.71 | 0.61 | –0.62 |
| 29 | chr28: 460266–462242 | ∗∗Putative glucose-regulated protein 78 | XP_001470161.1 | 207 | 75.2/71.94 | 5.5/5.05 | 1.55 | 0.04 |
| 30 | Yes | ∗∗Putative calmodulin | XP_001463554.1 | 50 | 55/70.47 | 5.6/4.41 | 0.45 | –0.54 |
| 31 | Yes | ∗∗S-adenosylmethionine synthetase | XP_003392704.1 | 73 | 48.5/43.12 | 6.1/5.49 | 0.45 | –0.08 |
| 32 | Chr30:122: 5029–1226102 | Conserved hypothetical protein | XP_001467184.1 | 115 | 30.2/40.83 | 5.9/5.32 | 0.11 | –0.16 |
| 33 | chr36: 1115280–1116995 | ∗∗Mitochondrial ATP-dependent zinc metallopeptidase, putative, metallo-peptidase, Clan MA(E), Family M41 | CCM19617.1 | 52 | 19.47/72.38 | 5.7/8.49 | 0.57 | –0.99 |
| 34 | chr11: 473373–474023 | Elongation factor 1-alpha | XP_003392396.1 | 112 | 52.36/49.12 | 6.8/9.03 | 0.61 | –1.31 |
| 35 | chr25: 548571–549221 | Putative GTPase | XP_001463009.1 | 223 | 12.4/24.24 | 5.9/6.09 | 0.29 | –0.31 |
∗Assembly location was calculated in reference to early passages. ∗∗These spots were located on both gels but identified once. Exp/Thr M.W., Experimental/Theoretical molecular weight. Exp/Thr pI, Experimental/Theoretical isoelectric point. Fold change, mean upregulation or downregulation with respect to early passage. aA value greater than 1.5 in column ‘a’ indicates up-regulation and less than 1/1.5 = 0.66 indicates down regulation. bNegative values indicates down regulation and positive values indicate upregulation.