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. 2018 Jul 3;9:865. doi: 10.3389/fpls.2018.00865

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Copyright © 2018 Ricachenevsky, Punshon, Lee, Oliveira, Trenz, Maraschin, Hindt, Danku, Salt, Fett and Guerinot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Yeast phenotype complementation assays. Empty pDR195 vector, pDR195-OsZIP7, pDR195-OsZIP4, and pDR195-OsZIP8 constructs were transformed into yeast cells. Liquid cultures were diluted as indicated before plating. OsZIP4 and OsZIP8 are positive controls (A) Zn-uptake defective strain zrt1zrt2 expressing OsZIP7 growing under Zn-sufficient (10 μM Zn) or Zn-deficient (no Zn added) conditions. (B) Zn-uptake defective strain zrt1zrt2 expressing OsZIP4 growing under Zn-sufficient (10 μM Zn) or Zn-deficient (no Zn added) conditions. (C) Zn-uptake defective strain zrt1zrt2 expressing OsZIP8 growing under Zn-sufficient (10 μM Zn) or Zn-deficient (no Zn added) conditions.