Time laps of the gelation process in a sugar-glass construct not loaded with CaCl2. From A through E, it is possible to see that the alginate mixture is leaking at the bottom of the construct over time (from 20 seconds to 21 minutes). Between 8 and 21 minutes (D) and (E), the sugar network completely dissolved itself, leaving only the outer walls. Thereby, for an alginate recipe requiring a certain laps of time, it is not possible to obtain intrinsic networks within an alginate scaffold. Scale bar: 10 mm.