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The Sequences of Gene and miRNA Primers Used for Real-Time PCR
| Genes | Primer Sequences (5′ to 3′) | Tm (°C) | |
|---|---|---|---|
| Has-miR-663a | AGGCGGGGCGCCGCGGGACCGC | 60 | |
| U6 | CGCTTCGGCAGCACATATAC | 60 | |
| NFIX | forward | CGGCTCTACAAGTCGCCTC | 60 |
| reverse | GCAGTGGTTTGATGTCCGC | ||
| GAPDH | forward | CAGGAGGCATTGCTGATGAT | 60 |
| reverse | GAAGGCTGGGGCTCATTT | ||
