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. Author manuscript; available in PMC: 2018 Jul 10.
Published in final edited form as: Nature. 2018 Apr 18;556(7702):501–504. doi: 10.1038/s41586-018-0052-z

Fig. 3. DI induces an Nrf2-independent response and inhibits the IL-6–IκBζ axis via ATF3.

Fig. 3

a, f, Western blots showing IκBζ expression in Nrf2−/− (a) and Atf3−/− (f) BMDMs. b, g, Cytokine levels in BMDMs treated with DI, stimulated with LPS for 4 h (b) and 24 h (g), mean ± s.e.m., n = 3 experiments. c, Genes regulated by DI independently of Nrf2. Hsp90, Ire1a and Perk are also known as Hsp84-2, Ern1 and Eif2ak3, respectively. ‘UPS response’ indicates UPS response pathways. d, Transcriptional comparison of Atf3−/− and wild-type BMDMs and enrichment of the Nrf2-independent DI signature. e, h, Western blots of ATF3 expression in BMDMs after DI treatment (e) and tolerized in the presence of BSO (h). Western blot data are representative of three experiments. For gel source data, see Supplementary Fig. 1. Statistical tests used were two-tailed t-tests. KO, knockout; NES, normalized enrichment score; UPR, unfolded protein response.