Fig. 4. DI inhibits IL-17-mediated IκBζ induction in keratinocytes and ameliorates psoriatic pathology.
a, b, Western blot of IκBζ expression in DI-treated, IL-17A-stimulated primary mouse (a) and human (b) keratinocytes. Representative of three mice or donors. For gel source data, see Supplementary Fig. 1. c, d, mRNA expression in DI-treated, IL-17A-stimulated (4 h) primary mouse (c) and human (d) keratinocytes, mean ± s.e.m., n = 3 mice or donors. e, Schematic of DI administration in a psoriasis model. f, Ear histology of control mice (left), IMQ-treated mice (middle) and mice treated with both IMQ and DI (right). Scale bars, 100 µm. Representative of six mice in two experiments. g, Quantification of the histology results in f, showing the relative change in ear thickness. Mean ± s.e.m., n = 6 mice. h, mRNA expression to show the induction of the indicated IκBζ target genes in ear tissue, mean ± s.e.m., n = 3 mice. Statistical tests used were two-tailed t-tests.