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. Author manuscript; available in PMC: 2018 Jul 10.
Published in final edited form as: Nature. 2018 Apr 18;556(7702):501–504. doi: 10.1038/s41586-018-0052-z

Extended Data Fig. 4. BSO potentiates the inhibitory effect of DI.

Extended Data Fig. 4

a, Western blot of Nrf2 expression in BMDMs treated with BSO or DI. b, GSH levels in BMDMs treated with BSO and stimulated with LPS. Mean ± s.e.m., n = 3 cultures. c, Cytokine production in BMDMs treated with BSO and stimulated with LPS. Mean ± s.e.m., n = 3 experiments. d, Cytokine production in BMDMs treated with DI and BSO and stimulated with LPS for 4 h. Mean ± s.e.m., n = 3 experiments. e, Cytokine production in BMDMs treated with 4EI (10 mM) and BSO and stimulated with LPS for 4 h. Mean ± s.e.m., n = 3 experiments. f, Western blot of IκBζ expression in BMDMs tolerized with LPS in the presence of BSO for 18 h and restimulated for 1 h (see Fig. 2l), asterisk shows the different exposures. Western blot data are representative of three experiments. For gel source data, see Supplementary Fig. 1. Statistical tests used were two-tailed t-tests.