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. 2018 May 8;27(3):520–530. doi: 10.1177/0963689717753186

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 9. — Flow cytometry (FCM) analysis of non-QQc PBMNCs and QQc PBMNCs. (A) Scatter diagrams of non-QQc PBMNCs and QQc PBMNCs by FCM analysis. The red lines indicate the cellular-sized gates of lymphocytes (a), monocytes (b), and the larger cells (c). (B) The graph shows the ratio of percent (%) cell positivity in QQc PBMNCs to that in non-QQc PBMNCs. n = 4 healthy volunteers. The investigated cell surface markers were as follows: hematopoietic stem cell (Cd34, CD133), endothelial cell (VEGFR-2, CD31, CD105), T cell (CD3, CD3/CXCR4/CD31), monocyte (CD14), M1 macrophage (CCR2), and M2 macrophage (CD206). Abbreviation: CCR2, CC chemokine receptor 2; FSC-A, forward scatter–area; SSC-A, side scatter–area; PBMNCs, peripheral blood mononuclear cells; QQc, quality and quantity control; VEGFR-2, vasular endotheilal growth factor recepor-2. *P < 0.05, **P < 0.01.