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. 2018 Jun 1;3(1):120–135. doi: 10.1089/can.2018.0010

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© Rameshprabu Nallathambi et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 3. — Determination of apoptosis or necrosis as cytotoxic effect of F7, F3, or F7+F3 on HCT 116 cells. HCT 116 cells were treated with F7 (20 μg/mL), F3 (36 μg/mL), the combination of F7 with F3 and solvent control (methanol) along with TNF-α (50 ng/mL) for 24 h (A) or 48 h (B). The treated cells were harvested and analyzed in FACS following Annexin V-FITC and PI staining. Shown are the percentages of live, necrotic, early, and late apoptosis cells, analyzed from 10,000 events per treatment. FACS, fluorescence-activated cell sorting; PI, propidium iodide.