Table 3.

Synergism Calculation for Combinations of Standards (THCA and CBGA)

	A. THCA (13.1 μg/mL)
	CBGA (53.3 μg/mL)	CBGA (40.0 μg/mL)	CBGA (28.0 μg/mL)	CBGA (20.0 μg/mL)	CBGA (13.3 μg/mL)	CBGA (6.7 μg/mL)
Calculated value	15.10	17.69	22.97	50.19	44.37	52.49
Experimental value	15.69	15.15	16.89	16.86	19.29	19.44

	B. CBGA (28.0 μg/mL)
	THCA (50.0 μg/mL)	THCA (30.0 μg/mL)	THCA (25.0 μg/mL)	THCA (15.0 μg/mL)	THCA (12.0 μg/mL)	THCA (6.0 μg/mL)	THCA (4.0 μg/mL)
Calculated value	10.53	20.43	21.25	44.66	47.60	70.59	69.18
Experimental value	16.56	14.40	16.90	23.25	28.69	29.68	42.44

(A) THCA in constant concentration of 13.1 μg/mL and CBGA in different concentrations (6.7–53.3 μg/mL). Italicized values are concentrations, which showed synergism between standards, as was determined by XTT assay on HCT 116 cells. (B) CBGA in constant concentration of 28 μg/mL and THCA in different concentrations (4.0–50.0 μg/mL). Italicized values are concentrations, which showed synergism between standards, as was determined by XTT assay on HCT 116 cells. The partial effect of the drugs was calculated according to the Bliss Independence Model for each combination experiment.

CBGA, cannabigerolic acid; THCA, tetrahydrocannabinolic acid.