Figure 4.

GAG and collagen II inhibited RANKL-induced NF-κB and MAPK pathways. BMMs were cultured in medium with M-CSF (30 ng/ml), the cells were co-cultured with DXM, with or without GAG and Collagen II for 24 hours, then cells were stimulated with RANKL (100 ng/ml) for 0 min, 15 min, 30 min and 60 min. The protein levels of several genes were quantified by western blotting. A. Phosphorylated expression of p65 (p-p65) increased in 15 minutes and then decreased in 1 hour after DXM stimulation. The level of p-p65 decreased overall with the treatment of GAG and Collagen II. B. The subfamilies of MAPK pathway (p-ERK, p-JNK, p-p38) presented a similar trend to phosphorylated expression of p65.