Figure 1.
Effect of CL097 stimulation on molecules involved in the MHC-I antigen presentation pathway. BMDCs (3×106/ml) were stimulated with 5 μg/ml GPC3, or 5 μg/ml GPC3 plus 0.5 μg/ml CL097 for 24 h. A-C. Determined by qRT-PCR assay, the mRNA expression levels of TAP1, TAP2, TAPBP, Bip, calreticulin, ERp57, ERAP, Sec22b, CD74, GILT, HSP90, EDEM1 and EDEM2 were calculated based on GAPDH. Bars show fold changes, with the GPC3-treated sample arbitrarily set as 1 (n=3). Data are presented as mean ± SE, Paired t-test was conducted between two groups. *P<0.05, **P<0.01. D. The concanavalin A agglutination assay was used to determine mannosylate residues (n=3).