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. 2018 Jun 15;10(6):1583–1599.

Figure 6.

Figure 6

Effects of Lv-Nurr1-MSCs on the number of nigral TH-positive neurons, the density of striatal TH-positive fibers, and the expression of Nurr1, TH and DAT in mRNA and protein level of the substantia nigra. The number of nigral TH-positive neurons and the density of striatal TH-positive fibers in the group of Lv-Nurr1-MSCs-injected were more than other group. TH-positive cells in the SN (A1-A4); TH-positive fiber in the striatum (The data has been shown in the text) (B1-B4); Representative RT-PCR of Nurr1, TH and DAT in the SN. GAPDH was used as an internal control (C). The relative mRNA level of Nurr1, TH and DAT was analyzed by RT-PCR (*P<0.01, **P<0.05) (D). Representative Western blot of Nurr1, TH and DAT protein in the striatum. β-actin was used as an internal control (E). The relative intensity of Nurr1, TH and DAT protein was analyzed by Western blot (Δ P<0.01, ΔΔ P<0.05) (F). (A) Substantia nigra; (B) Striatum; 1: normal side of the saline group; 2: saline-injected side of the saline group; 3: Lv-MSCs-injected side of the Lv-MSCs group; 4: Lv-Nurr1-MSCs-injected side of the Lv-Nurr1-MSCs group. The number of rats in each group was five. Scale bar: (A1-A4) = 100 μm; (B1-B4) = 50 μm.