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. 2018 Jun 15;10(6):1633–1647.

Figure 2.

Figure 2

Quantitative proteomic analysis in transfected HeLa cells. A. Experimental workflow for the quantitative proteomic analysis. Two pairs of pORF5-HeLa and HeLa cells (different passages) were analyzed as biological replicates. The samples were digested and labeled with the 113/115 and 114/116 iTRAQ tags, respectively. After separation by offline SCX LC, each fraction was analyzed by online RP NanoLC-MS/MS. Proteins were quantified using a cutoff of 1% global FDR. B. Numbers of identified and quantified proteins in the first and second runs. All of the proteins were consistent with an unused protein score of >1.3 and peptides of ≥2. C. Numbers of dysregulated proteins according to the protein quantification.