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. 2018 Apr 3;62(2):2890. doi: 10.4081/ejh.2018.2890

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©Copyright B. Ma et al., 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Distribution of NMSCs, B cells, and DCs in the PALS (a, c) and red pulp (b, d) of C57BL/6 mouse spleen. Antibodies against GFAP (green), B220 (red), and CD11c (blue) label mainly NMSCs, B cells, and DCs, respectively. c) High-resolution view of (a) to show the interaction of NMSCs and immune cells in PALS. d) High-resolution view of (b) to show the interaction of NMSCs and immune cells in red pulp; cyan arrows indicate a B220⁺ B cell closely associated with NMSC processes; white arrows show a B220^- CD11c⁺ DC closely apposed to NMSC processes; yellow arrows indicate a B220⁺ CD11c⁺ DC with a close association with NMSC processes. CA, central artery; SN, splenic nodule; PALS, periarteriolar lymphoid sheath. Objective lens: 40x. Each image is a maximal intensity projection of a Z-stack. Stack size: 6.0 μm; optical slice interval: 0.50 μm. Scale bar: 20 μm.