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. 2018 Jul 9;19:525. doi: 10.1186/s12864-018-4917-1

Fig. 1.

Fig. 1

Effects of EMS mutagenesis on M. hominis survival. Concentrations of M. hominis PG21 were determined after treatment with various concentration of EMS for 1.5 h and 3 h (a). Three independent experiments were performed. The percentage of killing was calculated (b). In accordance with the literature, a 75% killing rate corresponding to exposure to approximately 9 mg/ml EMS for 3 h was chosen to generate the M. hominis PG21 mutant library