Table 1.
Oligonucleotides used in this study
| Primer target and designation | Primer sequence | Product size (bp) |
|---|---|---|
| vaa genea | ||
| VaaPG21-FE | 5’-AATGGGATAGTTAGTAAAGTCGG-3’ | 1983 |
| VaaPG21-RE | 5’-AACCCTACCATTGTCGCTAAGA-3’ | |
| VaaPG21-FE-IRD700 | 5’-IRD700-AATGGGATAGTTAGTAAAGTCGG-3’ | 1692 |
| VaaPG21-RI-IRD800 | 5’-IRD800-GAAAAATCTCCTCGTGCTGATG-3’ | |
| oppA gene | ||
| OppAPG21-FE | 5’-GACAGTGGAAAAGCAGACAC-3’ | 1524 |
| OppAPG21-RE | 5’-AGGAACTAAAATGTCCGGGT-3’ | |
| OppAPG21-FI-IRD700 | 5’-IRDye700- CATCTTCTGGCCAAGCAACT − 3’ | 1144 |
| OppAPG21-RI-IRD800 | 5’-IRDye800-CAGTCTTGGTATGAATCAAC-3’ | |
aVaaPG21-FE and VaaPG21-RE primers are located upstream and downstream the vaa gene, respectively