Table 2.
vaa- and oppA-mutants obtained by screening the 1200 EMS-generated M. hominis clone library
| Mutant designation | Targeted gene | Nucleotide substitutiona | Amino-acid substitutionb | Comments |
|---|---|---|---|---|
| Mho66 | vaa | G410726A | Glu54Lys | |
| Mho119 | vaa | C411332T | Leu328Phe | |
| Mho787 | vaa | C410526A | Pro59His | |
| Mho864 | vaa | C410197T | none | Mutation located upstream of the vaa gene (intergenic region) |
| Mho1055 | vaa | C410154T | none | Mutation located upstream of the vaa gene (intergenic region) |
| Mho1133 | vaa | G410726A | Gly126Ser | |
| Mho258 | oppA | G181068A | Leu813Leu | Silent mutation |
| Mho369 | oppA | G180439A | Asp604Asn | |
| Mho785 | oppA | C181239T | Asp870Asp | Silent mutation |
| Mho940 | oppA | G180634A | Glu669Lys |
aNucleotide substitutions are numbered according to the M. hominis PG21 genome (GenBank accession number FP236530)
bAmino acid substitutions are numbered from the N-terminal end of the targeted protein