Table 3.
Mutations harboured by EMS-generated M. hominis mutants in comparison to the sequence of the M. hominis PG21 strain not exposed to EMS
| Mutant designation | Expected mutationa (gene, position in the gene) | Other mutations in the genomea (Locus, gene) | Number of additional mutations |
|---|---|---|---|
| Mho66 | G410726A (vaa, 376) | none | 0 |
| Mho119 | C411332T (vaa, 982) | - C230929T (intergenic) - C316577T (MHO_2640, CHP) - G463593A (MHO_3770, licA, Protein LicA homolog) - G494526A (MHO_3990, nusG, Transcription antitermination protein) |
4 |
| Mho787 | C410526A (vaa, 176) | - C5000T (MHO_0050, dnaN, DNA polymerase III beta chain) - C44025T (MHO_0340, ktrA, Potassium uptake protein KtrA) - C172081T (MHO_1460, rpsG, 30S ribosomal protein S7) - C258548T (MHO_2150, rpoD, RNA polymerase sigma factor) - C283090T (MHO_2320, ABC transporter, ATP-binding protein) - G288972A (MHO_2370, tRNA/rRNA methyltransferase) - C441828T (MHO_3650, asnA, Aspartate-ammonia ligase) - C571686T (MHO_4600, pyrH, Uridylate kinase) |
8 |
| Mho1133 | G410726A (vaa, 376) | - G179389A (MHO_1510, oppA) - C206418T (MHO_1700, recU, Recombination protein U homolog) - C381191T (MHO_3200, CHP, Hypothetical lipoprotein) - G462316A (MHO_3760, gyrB, DNA gyrase subunit B) - G490546A (intergenic) - C644067T (MHO_5190, pip, Proline iminopeptidase) |
6 |
| Mho369 | G180439A (oppA, 1813) | - G230779A (intergenic) | 1 |
| Mho940 | G180634A (oppA, 2008) | - G136127A (MHO_1080, ATB-binding protein) - G138607A (MHO_1090, HP) - G203092A (MHO_1670, cmk, Cytidylate kinase) - G211597A (MHO_1740, oppB, Oligopeptide transport system permease protein) - A227310G (MHO_1880, aspS, Aspartyl-tRNA synthetase) - G357674A (MHO_3060, leuS, Leucyl-tRNA synthetase) - G371725A (MHO_3130, atpA, F1-likeX0) - G649646C (MHO_5220, hsdS, Type I restriction enzyme specificity protein) |
8 |
amutations are numbered according to the M. hominis PG21 genome (GenBank accession number FP236530)
CHP conserved hypothetical protein, HP hypothetical protein