Figure 1.

Both painless and dTRPA1 mutants display no reduction in proboscis extension to allyl isothiocyanate (AITC). Frequencies of proboscis extension reflex (PER) are plotted for the w¹¹¹⁸, pain⁰¹, dTRPA1⁰¹ and pain⁰¹; dTRPA1⁰¹ double mutant lines for 0 mM, 1, 2, 5 and 10 mM AITC tested with different sucrose concentrations (1% plotted in panel A, 2% in Panel B, 5% in Panel C and 10% in Panel D). When tested at 1% sucrose, no differences in PER frequencies were observed at any AITC concentration for any genotype. At higher sucrose concentrations, higher PER frequencies were observed for the control solution (0 mM AITC) for each genotype. Addition of AITC lead to a significant decrease in PER frequency for the control genotype (w¹¹¹⁸), whereas in the other genotypes, no such decrease in PER frequency was observed. Numbers in the boxes refer to the total number of individual flies tested under the corresponding experimental condition. **Indicates significance at p < 0.001 (Tukey’s post hoc test, one-way analyses of variance (ANOVA)).