Figure 4.

Both painless and dTRPA1 mutants show no avoidance to an AITC point source. (A) Measurements of the average total duration in the AITC zone (defined as 5 cm (or halfway) from the AITC point source) are plotted for each genotype. In the control genotype (w¹¹¹⁸) with no AITC, the duration spent in one zone vs. the other is equal, whereas in the same genotype tested with AITC, the animals spent significantly less time in the AITC zone (P = 0.001 One Way ANOVA; each symbol represents a single animal trial and the lines represents the mean for all animals). For all the other genotypes, there was no significant difference in the time spent in one zone as compared to the other. (B) The average distance from the AITC point source (center line) and standard deviation are plotted for the same genotypes. The w¹¹¹⁸ animals tested with AITC were significantly different than 5 cm away (reflecting a random distribution; P = 0.001, One-Way T-Test), whereas the other gentoypes showed no significant difference from the center point.