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. 2018 Jul 3;12:45. doi: 10.3389/fncir.2018.00045

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Copyright © 2018 Mandel, Shoaf, Braco, Silver and Johnson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of the painless-GAL4 and dTRPA1-GAL4 lines appears to be expressed in different tissues. We introduced a membrane-tethered GFP variant under the control of the painless-GAL4 and dTRPA1-GAL4 drivers and examined different peripheral tissues and the central nervous system. Expression of painless-GAL4 in the labellum (right) is widely expressed in multiple sensory neurons (white arrows) compared to the dTRPA1-GAL4 (left) in the same structure, in which only a cluster of a few neurons were detectable (white arrow). Expression of painless-GAL4 (left) in the leg also appears to be widespread in multiple neurons in the distal portions of the leg (white arrows) compared to the dTRPA1-GAL4 (right) expression pattern that is more proximal and in fewer cells (white arrows).