Fig. 2.

Cortactin promoted the migratory and invasive potential of NSCLC cells in vitro. a A549 and H1299 cells were transfected with cortactin small interfering RNA (siRNA) or the siRNA control oligonucleotides, followed by qRT-PCR analysis of cortactin levels in the differently treated cells. b Protein expression levels of cortactin were measured by Western blotting. c Wound healing assay in A549 and H1299 cells transfected with cortactin siRNA or siRNA control oligonucleotides. After 32 h, wound healing assays were performed in triplicate. Representative images are displayed Scale bar, 800 μm, 4× magnification. The values shown are the mean ± SD, *P < 0.05. d Transwell migration and invasion assays of A549 and H1299 cells were performed after transfection with cortactin siRNA or siRNA control oligonucleotides. Representative figures of the migrated and invaded stained cells are shown on the left. Data are shown as the mean ± SD. Representative images are displayed on the right. Scale bar, 200 μm, 20× magnification, *P < 0.05