Fig. 7.

miR-182 inhibited the expression of Rock1 and Rac1 expression in NSCLC cells. a Rock1 and Rac1 expression was examined by Western blotting. A549 and H1299 cells were stimulated with 20 ng/mL HGF or 500 nM PDBu for the indicated time before cells were collected and lysed. β-Actin was used as a control. b A549 and H1299 cells were transfected with scrambled miRNA, miR-182, si-NC and CTTN siRNA, followed by treatment with or without HGF or PDBu. Cells were then subjected to western blot analysis to examine Rock1 and Rac1 levels. c Following treatment with 0,10, and 20 μM PP2, A549 and H1299 cells were harvested. Levels of Rock1 and Rac1 were determined using Western blot analysis. β-actin was used as a control