Table 1. Kinetic and thermodynamic parameters of binding between Ebola virus NPCts and MJ20 determined by surface plasmon resonance and isothermal titration calorimetry.
| NPCt | k on (M −1 s−1) | k off (s−1) | K d (nM) | ΔH (kcal mol−1) | −TΔS (kcal mol−1) | ΔG (kcal mol−1) | K d (nM) |
|---|---|---|---|---|---|---|---|
| EBOV | 1.7 × 105 | 7.1 × 10−4 | 4.1 | −31.15 | 19.65 | −11.50 | 3.7 |
| BDBV | 2.6 × 105 | 4.3 × 10−3 | 16.6 | −26.23 | 15.86 | −10.37 | 23.4 |
| TAFV | 3.7 × 105 | 1.1 × 10−3 | 3.1 | −24.61 | 13.24 | −11.37 | 4.5 |
| SUDV | 1.8 × 105 | 3.6 × 10−3 | 20.3 | −18.26 | 8.34 | −9.91 | 51.0 |
| RESTV | 1.9 × 105 | 2.2 × 10−3 | 11.7 | −29.32 | 18.49 | −10.83 | 11.6 |