Table 2. Crystallization information.
| Protein | FnNanE | VcNanE |
|---|---|---|
| Method | Vapour diffusion, hanging drop | Vapour diffusion, hanging drop |
| Plate type | 96-well plate | 96-well plate |
| Temperature (K) | 291 | 291 |
| Protein concentration (mg ml−1) | 18 | 28 |
| Buffer composition | 25 mM Tris pH 8.0, 50 mM NaCl, 6% glycerol, 10 mM BME | 20 mM HEPES pH 7.5, 100 mM NaCl, 5 mM BME |
| Reservoir composition | 0.1 M MES pH 5.2, 0.4 M MgCl2, 22% PEG 3350 | 0.2 M malonate pH 5.0, 20% PEG 3350, 1 M malonate pH 7.0 |
| Volume of drop | 200 + 200 nl | 500 + 500 nl |
| Volume of reservoir (µl) | 100 | 100 |