Table 2. Crystallization conditions.
| Method | Robot | Manual (hanging drop) |
|---|---|---|
| Plate type | Sitting-drop MDL3 plates | XRL hanging-drop 24-well MD3-11 plates |
| Temperature (K) | 293.15 | 293.15 |
| Protein concentration (mg ml−1) | 9.10 | 9.10 |
| Buffer composition of protein solution | 50 mM Tris–HCl pH 7.5, 150 mM NaCl | 50 mM Tris–HCl pH 7.5, 150 mM NaCl |
| Composition of reservoir solution | 0.1 M CHES pH 9.5, 20% PEG 8000 | 0.1 M CHES pH 8.5, 18% PEG 8000 |
| Volume and ratio of drop | 0.2 µl, 1:1 | 2 µl, 1:1 |
| Volume of reservoir (µl) | 40 | 500 |