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. 2018 Jun 26;74(Pt 7):385–390. doi: 10.1107/S2053230X18007689

Table 2. Crystallization.

Method Vapour diffusion
Plate type VDX plate (Hampton Research)
Temperature (K) 293
Protein concentration (mg ml−1) 3.3
Buffer composition of protein solution 100 mM Tris–HCl pH 8.2, 150 mM NaCl, 1 mM dithiothreitol, 7.7 mM AMP-PNP, 7.7 mM L-glutamic acid, 7.7 mM magnesium sulfate
Composition of reservoir solution 100 mM bis-tris propane pH 7.0, 210 mM sodium sulfate, 16% PEG 3350
Volume and ratio of drop 2 µl, 1:1 ratio
Volume of reservoir (µl) 500