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. 2018 Jun 26;74(Pt 7):425–430. doi: 10.1107/S2053230X18009093

Table 2. Crystallization.

Method Vapour diffusion, hanging drop
Plate type 24-well VDX plate without sealant
Temperature (°C) 22
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM Tris pH 6.8
Composition of reservoir solution 25% PEG 4000, 0.2 M MgCl2, 0.2 M NaCl, 0.1 M Tris–HCl pH 8.5
Volume and ratio of drop 4 µl (1:1)
Volume of reservoir (ml) 1