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. 2018 Jun 26;2018:1725191. doi: 10.1155/2018/1725191

Figure 4.

Figure 4

Propofol induced dephosphorylation and nuclear translocation of YAP in HT-22 cells. (a) HT-22 cells were starved for 16 hr, then treated with propofol (15 μmol/l) for different times or with different concentrations of propofol for 4 hr. Western blots were used to analyze the expression of phosphorylated YAP and total YAP. Representative results are shown from three independent experiments. aP < 0.01 versus 0 min, bP < 0.001 versus 0 min. (b) Propofol-induced (15 μmol/l for 4 hr) YAP nuclear translocation is shown in starved HT-22 cells by immunofluorescence staining and Western blots. Green: YAP; blue: DAPI. (c) Starved HT-22 cells treated with 15 μmol/l propofol for 12 hr, then mRNA and protein levels of survivin and Bcl2 were detected by real-time PCR and Western blot. aP < 0.05 versus control.