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. 2018 Jun 12;2018:2109352. doi: 10.1155/2018/2109352

Figure 4.

Figure 4

EGFR activation is required in Ang II-induced inflammation in vascular smooth muscle cells. After being pretreated with selective EGFR inhibitor AG1478 (5 μmol/l) for 30 minutes, vascular smooth muscle cells were then stimulated with 100 nmol/l Ang II for 24 hours. (a) EGFR activity, n = 4 independent experiments. (b) ERK1/2 activity, n = 3 independent experiments. (c–e) At the end of the experiment, the medium was collected to detect the concentration of IL-1β, IL-6, and TNF-α by ELISA, n = 3–5 independent experiments. (c) IL-1β protein level. (d) IL-6 protein level. (e) TNF-α protein level. (f) The mRNA levels of IL-1β, IL-6, and TNF-α, n = 3 − 4 independent experiments. Ang II represents angiotensin II. ∗ P < 0.05 versus control, ∗∗ P < 0.01 versus control, and # P < 0.05 versus Ang II.