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. 2018 Jun 26;2018:1978602. doi: 10.1155/2018/1978602

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Copyright © 2018 Tong Mu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Expression of neuron-specific markers before and after differentiation. (a) Immunofluorescence results for nestin, NSE, and MAP-2 in MSCs and MSCs-TFH1 before induction were negative. After induction, 3 markers in Neurons and Neurons-FTH1 exhibited strong positive expression. (b)-(c) qRT-PCR demonstrated that nestin, NSE, and MAP-2 were not significantly expressed in MSCs and MSCs-FTH1 before induction. After differentiation induction, nestin, NSE, and MAP-2 were significantly expressed in Neurons and Neurons-FTH1, and no significant differences were noted between these two groups (P>0.05).