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. 2018 Jul 3;9:896. doi: 10.3389/fpls.2018.00896

FIGURE 4.

FIGURE 4

Stress tolerance in yeast cells overexpressing GhSNAP33. (A) RT-PCR analysis of GhSNAP33 expression in yeast cells. EV, yeast cells transformed with the pYES2.0 empty vector; #1, #2, and #3, yeast cell lines transformed with the pYES2.0-GhSNAP33 vector. (B) Growth serially diluted yeast cells (1/10, 1/100, 1/500, and 1/1000 dilutions). (C) Growth of yeast cells (1/10, 1/100, 1/500, and 1/1000 dilutions) after 1 mM H2O2 treatment. (D) Growth of yeast cells (1/10, 1/100, 1/500, and 1/1000 dilutions) after 0.5 M mannitol treatment. These assays were repeated three times with similar results.