FIGURE 8.

Elevated callose deposition and reduced mycelia growth in GhSNAP33 transgenic Arabidopsis. (A) V. dahliae spores suspension was applied to detached WT and GhSNAP33 transgenic plants; representative leaves are shown at 6 dpi. (B) Lesion area of infected plants at 6 dpi. (C) The mycelia growth and cell death on infected leaves at 6 dpi stained with trypan blue. Top panel: Trypan blue staining of leaves. Lower panel: Closeups from corresponding top panel. The fungal mycelia are indicated by red arrows. (D) Callose deposition on the infected leaves stained with aniline blue at 24 h after inoculation. Similar results were obtained in independent experiments. Data represent mean ± standard error of three independent repeats (n = 3). ^∗∗P < 0.01 vs. WT.