Fig. 12.2.

Distributions of integrin α_IIbβ₃ conformers in the presence and absence of extracellular ligands (RGD), cytosolic binding partners (talin head domain F3) and lipid bilayer nanodiscs. Between two and four independent data sets were acquired for each condition. The standard deviation for the percentages was below 2 percentage points for all measurements (see error bars). The presence of talin head domain shifts the equilibrium slightly by 4.7% towards the upright conformation with separated legs. The trend is somewhat reversed if RGD is added as well. When nanodiscs are added, there is a major shift of 14.8% towards the upright (iv) conformation