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. 2018 Jul 4;11:3805–3815. doi: 10.2147/OTT.S162700

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© 2018 Lin et al. This work is published and licensed by Dove Medical Press Limited

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c-jun silencing inhibited the migration and invasion of CNE-2R cells both in vitro and in vivo.

Notes: (A–C) Scratch assay and transwell assay to compare the cells migration and invasion in c-jun-shRNA group, NC group, and control group in vitro. (C) The establishment of the nude mice model and the demonstration of metastasis of CNE-2R cells in the lung. (I) Nude mice used in the experiment. (II) The formation of metastatic tumor in the lung. The differential formation of metastatic tumors in the lungs of the three groups after 6 weeks, the number of metastatic tumors in the lungs of c-jun-shRNA group was significantly lower than that of NC group and control group (**P<0.01). (III) Microscopic analysis of the lung metastatic tumors by H&E staining in the three cell groups. The level of pulmonary metastasis of c-jun-shRNA group was significantly lower than that of NC group and control group (**P<0.01). Control represents non-transfected group and NC represents scrambled shRNAtransfected group. Magnification ×100.

Abbreviation: NC, negative control.

c-jun silencing inhibited the migration and invasion of CNE-2R cells both in vitro and in vivo.

Notes: (A–C) Scratch assay and transwell assay to compare the cells migration and invasion in c-jun-shRNA group, NC group, and control group in vitro. (C) The establishment of the nude mice model and the demonstration of metastasis of CNE-2R cells in the lung. (I) Nude mice used in the experiment. (II) The formation of metastatic tumor in the lung. The differential formation of metastatic tumors in the lungs of the three groups after 6 weeks, the number of metastatic tumors in the lungs of c-jun-shRNA group was significantly lower than that of NC group and control group (**P<0.01). (III) Microscopic analysis of the lung metastatic tumors by H&E staining in the three cell groups. The level of pulmonary metastasis of c-jun-shRNA group was significantly lower than that of NC group and control group (**P<0.01). Control represents non-transfected group and NC represents scrambled shRNAtransfected group. Magnification ×100.

Abbreviation: NC, negative control.