Table 2.
Primers used in qPCR analysis
| Gene name | Primer sequence | PCR product size (bp) |
|---|---|---|
| GAPDH | F: TCACCATCTTCCAGGAGCG; R:CTGCTTCACCACCTTCTTGA | 572 |
| SCX | F: CGTTGCCCAGGTGCGAGATGTAG; R: CGAGCGAGACCGCACCAACA | 144 |
| TNMD | F: TTGAAGACCCACGAAGTAGA; R: ATGACATGGAGCACACTTTC | 170 |
| COL1 | F: GGCGGCCAGGGCTCCGACCC; R: AATTCCTGGTCTGGGGCACC | 347 |
| COL3 | F: TGGTGTTGGAGCCGCTGCCA; R: CTCAGCACTAGAATCTGTCC | 373 |
| COL6 | F: CCCTGAGGCTTAACTTGCTG; R: CCAGGATTCCCTTTCTCTCC | 388 |
| Tenascin-C | F: TGACAGAAGTGACGGAAGAG; R: GATGGCAAATACACGGATAA | 194 |
| Decorin | F: AATAACCGAAATCAAAGATGGAGAC; R: TCAGCAATGCGGATGTAGGAG | 351 |
| BGN | F: GGTGGCTAGGTCTCCCCTTA; R:CACGTTGCACGGTGTTTCTT | 99 |
| FMOD | F: TTTATAGCGTCAGGCGGACC; R:GTCCCGCCCCAAATTCCTAA | 135 |
| Runx2 | F: ACAGTAGATGGACCTCGGGA; R:ATACTGGGATGAGGAATGCG | 113 |
| OCN | F: ATGAGAGCCCTCACACTCCTCG; R: GTCAGCCAACTCGTCACAGTCC | 255 |
| ALPL | F: AAACCGAGATACAAGCACTCCCAC; R:TCCGTCACGTTGTTCCTGTTCAG | 140 |
| SOX9 | F: AGCCGAAAGCGGAGCTCGAAACT; R:GCACTTAGGAAGGCGCGGGGT | 215 |
| Aggrecan | F: AGTATCATCAGTCCCAGAATCTAGCA; R: AATGCAGAGGTGGTTTCACTCA | 132 |
| COL2A1 | F: CTTGGGCACCTCGGGCTCCTTTAG; R: TCCCCGGCACTCCTGGCACTGAT | 510 |
| PPARγ | F: AGCCTCATGAAGAGCCTTCCA; R: ACCCTTGCATCCTTCACAAGC | 110 |
| C/EBPα | F: AAGAAGTCGGTGGACAAGAACAG; R: TGCGCACCGCGATGT | 70 |
| AP-2α | F: AGGGCGAAGTCTAAAAATGGAG; R: TAGTGATGTGAGCAGGGTAACG | 114 |
| Adiponectin | F: TATCCCCAACATGCCCATTCG; R: TAGGCAAAGTAGTACAGCCCA | 114 |
| MyoD | F: CGGCGGAACTGCTACGAAG; R: GCGACTCAGAAGGCACGTC | 172 |
| Myogenin | F: CAACCAGGAGGAGCGAGACCTCCG; R: AGGCGCTGTGGGATATGCATTCACT | 86 |
Abbreviations: ALPL, alkaline phosphatase, liver/bone/kidney; AP-2α, activating enhancer binding protein 2α; BGN, biglycan; C/EBPα, enhancer binding protein α; COL1, type I collagen; COL2A1, type II collagen A1; COL3, type III collagen; COL6, type VI collagen; FMOD, fibromodulin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MyoD, myoblast determination protein; OCN, osteocalcin; qPCR, quantitative PCR; PPARγ, peroxisome proliferator-activated receptor-γ; Runx2, Runt-related transcription factor 2; SCX, scleraxis; SOX9, SRY (sex determining region Y)-box 9; TNMD, tenomodulin.