Table 3.

Different FA concentrations compete with PFP/PLGA-PEG-FA binding of SKOV3 cells (n=3)^*

FA concentrations (pg/mL)	Positive cells (%)
0	99.77±0.40
25	47.60±2.98‡,§,¶
50	14.52±5.19‡,§
100	5.67±1.99‡,¶
200	0.39±0.19‡
400	0.68±0.16‡
800	0.24±0.06‡

Notes:

^*SKOV3 cells were treated with 10 μL of FA with different concentrations, followed by incubation in nitrogen dioxide for 30 min at 37°C, 40 μL of 64×10⁴ particles/mL of PFP/PLGA-PEG-FA was then added and incubated in nitrogen dioxide at 37°C for another 30 min.

^‡Comparison to control group (without FA), P<0.01;

^§Comparison between 2 groups, P<0.05;

^¶Comparison between 2 groups. P<0.01.

Abbreviations: FA, folic acid; PEG, polyethylene glycol; PFP, perflenapent; PLGA, poly lactic-co-glycolic acid.