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. Author manuscript; available in PMC: 2018 Jul 10.
Published in final edited form as: Nat Ecol Evol. 2017 Sep 11;1(10):1535–1542. doi: 10.1038/s41559-017-0285-5

Figure 2. Fate mapping reveals an ectodermal origin of the pharynx and septal filaments.

Figure 2

Schematized transplantation experiment and fate map (a, b), single confocal images (c, d, e-e”, f, g) and schematics (c’, d’, f’, g’) of cryo-sectioned animals containing transplanted tissue (c-g’). Samples are exemplary primary polyps (c-d’, f-g’) or a juvenile polyp (e-e”) with pure ectodermal (c-e”) or pure endodermal (f-g”) integration of transplanted, mem-Orange-positive donor tissue. A detailed description of the transplantation techniques and results is provided in Supplementary Figure 1. Colours in confocal pictures: yellow: mem-Orange staining; magenta: F-actin staining; blue: nuclear staining (DAPI). bw: body wall; ec: ectoderm; en: endoderm; mes: mesentery; ph: pharynx; sep fil: septal filament; som gon: somatic gonad. Scale bars in c, d, f, g: 20μm; in e: 200μm; in e’, e”: 25μm.