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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Biomaterials. 2018 May 25;176:122–130. doi: 10.1016/j.biomaterials.2018.05.043

Figure 4. In vitro characterization of aPACE polymers (HEK293 cells).

Figure 4

(a) Luciferase mRNA transfection efficiency depending on the actuation time demonstrating an optimal actuation time for each MW. (b) Transfection efficiency using non-actuated PACE and actuated PACE of different initial MW at their optimal actuation time (5 days for the 5 kDa polymer, 10 days for the 10 kDa polymer, and 30 days for the 20 kDa polymer, **p < 0.005). (c) Cytotoxicity profiles of mRNA:aPACE polyplexes compared to the mRNA:TransIT complexes. (d) Transfection efficiency of aPACE using different temperature of actuation (**p < 0.005). All results are presented as mean ± SD of three independent experiments run in duplicate.