(a) EPO blood concentration 6 h after IV administration of
mRNA (20 µg total) using TransIT, 5 kDa non-actuated PACE, 5 kDa aPACE
actuated for 5 days, 10 kDa non-actuated PACE, or 10 kDa aPACE actuated for 10
days. Results are presented as mean ± SD of N = 3 animals (****p
< 0.0001). (b) Time course of EPO production following IV
administration of mRNA (20 µg total) using TransIT, 5 kDa non-actuated
PACE, 5 kDa aPACE actuated for 5 days, 10 kDa non-actuated PACE, or 10 kDa aPACE
actuated for 10 days. Results are presented as mean ±SD of N = 3 animals
(***p < 0.001 and *p < 0.05). (c) Blood chemistry analysis 24 h
and 7 days after IV administration of acetate buffer, free mRNA or mRNA:aPACE
polyplexes. Results are presented as mean ± SEM of N = 3 animals.
(d) Histology analysis of liver and spleen 24 h after IV
administration of acetate buffer or mRNA:aPACE polyplexes. Images are
representative of N = 3 animals.