. Author manuscript; available in PMC: 2019 May 1.

Published in final edited form as: J Biomed Mater Res A. 2018 Jan 10;106(5):1177–1188. doi: 10.1002/jbm.a.36315

TABLE I.

Composition of CD4 Binding Ligands on LCNPs

LCNP formulations	Feed mass ratio (wt %)	Feed ligand molecules per LCNP^a	Ligand loading (wt %)^b	Average ligand molecules per LCNP^a	Conjugation efficiency (%)^b
BP4-dtLCNP	1.24	11,126	1.22 ± 0.01	10947	98.4 ± 0.2
CD4-dtLCNP	10.17	2053	4.17 ± 0.10	842	41.0 ± 1.0
fCD4-dtLCNP	10.17	2053	6.20 ± 0.07	1250	60.2 ± 0.7
BP4-cbLCNP	1.24	11,126	1.23 ± 0.01	11070	99.5 ± 0.2
CD4-cbLCNP	10.17	2053	4.36 ± 0.10	881	42.9 ± 0.9
fCD4-cbLCNP	10.17	2053	6.28 ± 0.04	1268	61.0 ± 0.5

The number of fCD4 molecules was regarded as the number of full antibodies that generated these fCD4 (one antibody could generate 2–4 fCD4 by TCEP reduction).

Data represents mean ± SD from at least three samples.