Figure 4.

Analysis of NGF concentration-dependent oligomeric equilibrium [initial state; dimer model (PDB entry 1btg); intermediate state, unknown oligomer; final state, set to ‘none’]. The top left panel displays the experimental data (dots with error bars), and the DAMMIX fits as red solid lines. The previously reported models (dimer and head-to-head dimer of dimers) are shown in the bottom right panel with magenta and green C_α traces (Covaceuszach et al., 2015 ▸). A typical restored shape of the unknown component obtained by DAMMIX is displayed in the bottom right panel with red beads. The scale bar is 20 Å. The scattering curves from the components are shown in the bottom left panel (the two most different restored curves for the intermediate obtained from multiple DAMMIX runs are shown with dashed red lines) and their restored volume fractions are displayed in the inset; the colours are the same as the bottom right panel and the error bars of volume fractions display the average dispersion over multiple DAMMIX runs.